RESUMO
BACKGROUND: We report results of the entomo-virological surveillance system in Aedes aegypti local populations performed by the Ministry of Health of Guerrero. METHODS: Indoor-adult Ae. aegypti collected at Acapulco, Zihuatanejo, Coyuca de Benitez and Atoyac de Alvarez (dry season, 2015) were processed for dengue virus (DENV) and chikungunya virus (CHIKV) using RT-PCR. RESULTS: We identified different seroptypes of DENV (2, 3 and 4), CHIKV and their co-circulation in field-caught mosquitoes across a significant geographic area. Pools of males were positive for CHIKV and DENV 3 and 4 suggesting vertical transmission. CONCLUSIONS: Entomo-virological surveillance in Guerrero has identified early circulation of CHIKV and DENV and provided a trigger for timely and focalized vector control actions.
Assuntos
Febre de Chikungunya/transmissão , Vírus Chikungunya/isolamento & purificação , Vírus da Dengue/isolamento & purificação , Dengue/transmissão , Transmissão Vertical de Doenças Infecciosas/estatística & dados numéricos , Aedes/virologia , Animais , Febre de Chikungunya/epidemiologia , Febre de Chikungunya/genética , Vírus Chikungunya/genética , Estudos Transversais , Dengue/epidemiologia , Dengue/genética , Vírus da Dengue/genética , Humanos , Insetos Vetores , México/epidemiologia , Reação em Cadeia da Polimerase em Tempo Real , Vigilância de Evento SentinelaRESUMO
We carried out dengue (DENV) and chikungunya virus (CHIKV) surveillance in wild populations of Aedes aegypti from Guerrero, Mexico, from 2012 to 2014 following a standard national protocol of the Mexican Dengue Control Program. A total of 284 pools (15-30 specimens/pool) of female mosquitoes were tested with real-time reverse transcriptase-polymerase chain reaction to detect DENV and CHIKV. We report for the 1st time the detection of CHIKV from field-collected mosquitoes at Acapulco and Juchitán in 2014. Results from DENV are also reported.
